LOCUS       pLV-[hsa-miR-502]        9140 bp    DNA     circular     27-MAY-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7061..7921
                     /label=Amp(R)
     promoter        8122..8662
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5666..5900
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6475..6930)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5972..6102
                     /label=SV40 pA
     misc_feature    6103..6316
                     /label=SV40 ori
     misc_feature    4987..5583
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3605
                     /label=intron
     CDS             3652..4977
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3395
                     /label=hsa-mir-502 precursor
BASE COUNT     2165 a      2387 c      2476 g      2112 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcca tatacacaat 
     3121 gcaagactct cattcctgtt ctcacacaga gctgtgtaac agagaacctt ctctgcttcg 
     3181 agcacggcag gtgcccaagc cttctgccct gctccccctc tctaatcctt gctatctggg 
     3241 tgctagtgct ggctcaatgc aatgcacctg ggcaaggatt cagagagggg gagctcaact 
     3301 gtaccaagaa ggacagagag cctagacatc acccctccca ccctcccctg tcatagtttt 
     3361 tgagtgatgg gtgtgtgttg gggaagcaca gaacagctag ctcgagcttt tggagtacgt 
     3421 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg 
     3481 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg 
     3541 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat 
     3601 ttcaggtgtc gtgaaaacta cccctctaga gtcgagctac cggtcgccac catggtgagc 
     3661 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg 
     3721 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac 
     3781 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg 
     3841 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac 
     3901 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac 
     3961 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc 
     4021 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag 
     4081 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag 
     4141 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag 
     4201 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 
     4261 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 
     4321 ggccgccact ccaccggcgg catggacgag ctgtacaagg acccaccggt cgccaccatg 
     4381 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 
     4441 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 
     4501 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 
     4561 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 
     4621 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 
     4681 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 
     4741 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 
     4801 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 
     4861 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 
     4921 gtcgagtgcc cgaaggaccg cgcgacctgg tgcatgaccc gcaagcccgg tgcctgagcg 
     4981 gccgcgtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 
     5041 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 
     5101 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 
     5161 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 
     5221 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 
     5281 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 
     5341 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc 
     5401 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 
     5461 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 
     5521 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 
     5581 ctggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 
     5641 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga 
     5701 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 
     5761 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 
     5821 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 
     5881 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 
     5941 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta 
     6001 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 
     6061 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc 
     6121 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 
     6181 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 
     6241 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt 
     6301 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 
     6361 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 
     6421 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc 
     6481 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 
     6541 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 
     6601 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 
     6661 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 
     6721 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 
     6781 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 
     6841 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 
     6901 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg 
     6961 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 
     7021 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 
     7081 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 
     7141 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 
     7201 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 
     7261 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 
     7321 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 
     7381 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 
     7441 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 
     7501 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 
     7561 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 
     7621 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 
     7681 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 
     7741 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 
     7801 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 
     7861 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 
     7921 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 
     7981 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 
     8041 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 
     8101 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 
     8161 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 
     8221 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 
     8281 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 
     8341 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 
     8401 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 
     8461 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 
     8521 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 
     8581 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 
     8641 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 
     8701 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 
     8761 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 
     8821 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc 
     8881 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 
     8941 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 
     9001 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 
     9061 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag 
     9121 ggaacaaaag ctggagctgc 
//
